Dietary SPI regulation of molecules related to breast CSC survival, inflammation, and metastasis in MaSC-enriched (CD29hiCD24+Lin) epithelial subpopulation within hyperplastic mammary tissues fromWnt1-Tg mice. (a) Molecular network from pathway analysis indicating dietary SPI suppression of molecules involved in MaSC and/or breast CSC survival, inflammatory/chemokine network, and migration/metastasis. Green indicates downregulation. QPCR analyses of transcripts for Il6
(b), Stat1
(c), Ccl8
(d), and Tnc
(e) in MaSC-enriched population (n=4–6 independent samples, where each sample represents RNAs pooled from 2–3 mice per diet group) as a function of dietary CAS or SPI exposure. 18s rRNA was used as normalizing control; *P≤0.05 relative to CAS. (f) IL6 levels were quantified in sera of PND75 Wnt1-Tg mice fed CAS or SPI; n=13 mice per diet group; * P ≤ 0.001 relative to CAS.