Table 1.
Oligonucleotide sequences of the primers used for RT-PCR
| Gene | Oligonucleotide Primers | Product(bp) | |
|---|---|---|---|
| TRPM1 | Sense | 5’-CCTCCTGTGGACACCAAAGT-3’ | |
| Antisense | 5’-AAGACAAGAGCGTCCAGCAT-3’ | 144 | |
| TRPM2 | Sense | 5’-CCATGCCACACATCAAACTC-3’ | |
| Antisense | 5’-ATGATGGCCCAAATGAGAAG-3’ | 121 | |
| TRPM3 | Sense | 5’-ACCGGGTTGACTTTGTGAAG-3’ | |
| Antisense | 5’-CAGATGAAGGGTGTTTGGT-3’ | 105 | |
| TRPM4 | Sense | 5’-ACCGGGTTGACTTTGTGAAG-3’ | |
| Antisense | 5’-GCAGATGAAGGGTGTTTGGT-3’ | 141 | |
| TRPM5 | Sense | 5’-GGCACACCCATCTTACGACT-3’ | |
| Antisense | 5’-ACTGTCTGGCTCCTGCAGAT-3’ | 126 | |
| TRPM6 | Sense | 5’-AAGCCATCCTGTCACCAAAG-3’ | |
| Antisense | 5’-CAAAGAGGTAGACGGCTTGC-3’ | 180 | |
| TRPM7 | Sense | 5’-GGAAGGTGTGTGCAAAATGA-3’ | |
| Antisense | 5’-GCTTCAGGTCCTTCACAAGC-3’ | 136 | |
| TRPM8 | Sense | 5’-GCTACGGACCAGCATTTCAT-3’ | |
| Antisense | 5’-GCTTGTCAATGGGCTTCTT-3’ | 172 | |
| β-actin | Sense | 5’-CCCATCTATGAGGGTTACGC-3’ | |
| Antisense | 5’-TTTAATGTCACGCACGATTTC-3’ | 150 |