Table 2.
Reagents used to mark or inhibit different types of endocytosis.
| Endocytosis Pathway |
Marker of Endocytosis |
Inhibitor of Endocytosis | Mechanism of Inhibitor |
|---|---|---|---|
| macropinocytosis | dextran | 5-(N-ethyl-N- isopropyl)amiloride (EIPA) |
Ion-channel inhibitor |
|
caveolae- mediated |
cholera toxin subunit B (CTXB) |
methyl-β-cyclodextrin (MβCD) |
Extracts cholesterol from the plasma membrane and disrupts formation of lipid rafts |
|
clathrin- mediated |
transferrin | chlorpromazine (chlor) |
Prevents assembly of clathrin coated pits at the plasma membrane |