Functional enrichment of genes that are overexpressed in 4th instar larvae from laboratory colonies of An. coluzzii and An. gambiae. A, An. coluzzii CAM from Yaoundé, Cameroon compared to An. gambiae Pimperena from Mali. B, Comparison of two colonies from Mali: An. coluzzii Mali-NIH and An. gambiae Pimperena. The numbers of significant genes for each DAVID annotation cluster are indicated above each bar. Enrichment scores are displayed within each bar. CYP450, cytochrome P450; UGT, UDP-glucuronosyltransferase; CE, carboxylesterase; GST, glutathione S transferase; PI, peptidase inhibitor; TEP, thioester-containing protein; Redox, oxidoreductase; CTL, C-type lectin.