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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Acta Neuropathol. 2014 Feb 14;127(6):861–879. doi: 10.1007/s00401-014-1256-4

Fig. 3. Immunization with AFF 1 reduced α-syn load in mThy1-α-syn tg mice.

Fig. 3

α-syn levels were measured in non-tg mice and mThy1-α-syn tg mice immunized either with vehicle or AFF 1. (a) α-syn immunostaining of substantia nigra using the α-syn antibody LB509 (green). Cell nuclei were stained with DAPI (blue). Scale bar = 10 μm. (b) Quantification of the percentage of neuropil area positive for α-syn in substantia nigra. (c) α-syn immunostaining of striatum using the α-syn antibody LB509 (green). Cell nuclei were stained with DAPI (blue). Scale bar = 10 μm. (d) Quantification of the percentage of neuropil area positive for α-syn in striatum. (e) Tyrosine hydroxylase (TH) immunostaining of striatum. Scale bar = 10 μm. (f) Quantification of the percentage of neuropil area positive for TH in striatum. (g) Immunoblot analysis of α-syn species (oligomers, dimers, and monomers). Levels of β-syn did not change with any of the treatments. β-actin was used as loading control. (h) Densitometric analysis of immunoblot results. (i) ELISA analysis of total levels of human α-syn. Results are expressed as average ± SEM. (*) p<0.05