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53BP1 and γ-H2AX colocalize in persistent or spontaneous DNA damage foci in MEFs. MEFs grown on glass slides were mock-irradiated or irradiated with 10 Gy X-rays and let recover for 48 h before fixation and staining with the indicated antibodies. Note that the yellow foci in the merge panel demonstrate co-localization between 53BP1 (red) and γ-H2AX (green).
