Fig. 2.

Image processing and masking. A) Raw image capture in grayscale. B) Same image as a pseudocolor heatmap. C) Raw image was deconvolved using the no-neighbors deconvolution deblurring algorithm, which attenuates lower intensities in the image and preserves spatial fidelity. D) No-neighbors deconvolved image was masked using our iterative masking approach (white outlines). E) Raw image was deconvolved using the constrained iterative deconvolution algorithm, which accurately restores the signal source in the image using the point-spread function for our imaging system. F) Mask generated in D overlaid on the constrained iterative deconvolved image. Adjacent color bars reflect grayscale levels images captured at 60x magnification.