Table 2.
Parameters derived from SANS for the PFV Intasome (see also Table S3)
| Source | H2O:D2O | Concentration (mg/mL) | Rg (Å)b | Dmax(Å)b |
|---|---|---|---|---|
| NIST NG-3 (SANS) | 0% D2O | 6.7 | 51.6 ± 0.3 | 155 |
| 0% D2O | 3.9 | 49.1 ± 1.6 | 155 | |
| 20% D2O | 7.3 | 53.7 ± 2.5 | 175 | |
| 65% D2O | 8.3 | 52.9 ± 0.6 | 160 | |
| 80% D2O | 7.7 | 53.0 ± 0.3 | 165 | |
| 90% D2O | 7.8 | 51.3 ± 0.3 | 160 | |
| 90% D2O | 3.9 | 49.1 ± 0.6 | 160 | |
| Stuhrmann Relationshipc | std. err. | |||
| Rc (Å) | 50.8 | ± 0.9 | ||
| Protein Rg (Å) | 53.7 | ± 1.5 | ||
| α | 1.2 | ± 158.4 | ||
| β | −473.8 | ± 323.0 | ||
| Parallel Axis Theoremc | ||||
| Protein Rg (Å) | 56.6 | ± 3.4 |
a
Determined by Bradford protein assay
b
Determined by GNOM analysis (Semenyuk and Svergun, 1991)
c
Determined by MuLCH analysis (Whitten et al., 2008)