Figure 5.

ROS generation was critical for PL-induced apoptosis in OVCAR3 ovarian cancer cells. (a) ROS accumulation and (c) cell apoptosis in PL-treated OVCAR3 cells were reversed by NAC. Cells were treated with PL (10 μM) for 24 hr in the presence or absence of 3 mM NAC pretreatment for 1 hr. The apoptosis was detected by FCM with Annexin V/PI staining and the expression of C-PARP proteins was analyzed by Western blot. (c) The quantified results of (d). (b) Representative Western blotting analysis of C-PARP in OVCAR3 cells treated as indicated. β-actin was used as loading control. *P < 0.05 and **P < 0.01 versus corresponding control.