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. Author manuscript; available in PMC: 2014 May 27.
Published in final edited form as: Wound Repair Regen. 2007 May-Jun;15(3):413–421. doi: 10.1111/j.1524-475X.2007.00244.x

Figure 3.

Figure 3

Alkaline phosphatase (ALP) activity of human mesenchymal stem cells (hMSCs) osteogenic cultures with various doses of transforming growth factor β-3 (TGFβ3) released from poly(DL-lactic-co-glycolic acid) (PLGA) microspheres (Experiment 1). Increased activity of ALP, an early marker for osteogenic differentiation up to 3 weeks in osteogenic culture was observed. Various doses of TGFβ3 (3 mg of low-concentration spheres: 0.035 ng/mL released after 1 week; 3 mg of high-concentration spheres: 0.135 ng/mL released after 1 week; and 30 mg of high-concentration spheres: 1.35 ng/mL released after 1 wk) inhibited increases in ALP activity up to 3 wks, indicating inhibition of osteogenic differentiation of hMSCs (n=3, p < 0.05).