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. Author manuscript; available in PMC: 2014 May 27.
Published in final edited form as: Wound Repair Regen. 2007 May-Jun;15(3):413–421. doi: 10.1111/j.1524-475X.2007.00244.x

Figure 5.

Figure 5

Dose dependence of human mesenchymal stem cells (hMSCs) to transforming growth factor β-3 (TGFβ3) in osteogenic culture (Experiment 1). Red staining of alkaline phosphatase (ALP) and black coloring of mineral deposition by von kossa stain were progressively increased in osteogenic cultures of groups with no TGFβ3 (0 ng/mL) over the 28-day period (A → B → C). Groups with highest dose of released TGFβ3 (1.35 ng/mL in the first week) had less staining intensity of both von kossa and ALP up to 28 days of osteogenic culture, indicating the inhibition of osteogenic differentiation of hMSCs. Negative controls were hMSCs cultured in non-osteogenic growth medium.