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. Author manuscript; available in PMC: 2014 May 27.
Published in final edited form as: Nat Protoc. 2012 Jun 7;7(7):1247–1259. doi: 10.1038/nprot.2012.051

Figure 6.

Figure 6

Characterization of diffusion profile of applied VEGF. (a) 4 hours of VEGF diffusion into type 1 collagen is simulated (left). Diffusion coefficient of VEGF in type 1 collagen in the simulation was 5.8×10-11 m2/sec. Arrows indicate diffusive flux. Steady-state concentration profile is shown along the black line (right). Blue color corresponds to 0 ng/ml VEGF while red corresponds to 50 ng/ml VEGF. (b) Gradient (left) and normalized intensity plot from 0 to 30 minutes (center) and from 30 minutes to 10 hours (right). Intensity was measured from right to left sides of type 1 collagen (2.0mg/ml, polymerized at pH 7.4), and then normalized to unity in each figure. (c) Angiogenic response from ECs monolayer was induced by the VEGF diffusion gradient from the right channel (red arrowhead). (d) Perimeter, area and length of angiogenic response were measured (segmented in pale red) and quantified in the captured images.