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SAH levels are increased in cells exposed to the combination of adenosine and homocysteine. HepG2 cells were incubated for 2 hours in medium alone (DMEM) or in medium supplemented with 500 µM adenosine (Ado), 500 µMhomocysteine (Hcy), or the combination (Ado+Hcy). Intracellular SAH was measured by HPLC as described in Materials and Methods. Data represent the means ± sd of 3 independent measurements.
