Fig. 4.

TNF-induced degradation of IκB-α and nuclear translocation of NFκB is not inhibited by SAH. HepG2 cells were pre-treated for 2 hours with either medium alone (DMEM) or the combination of 500 µM adenosine and 500 µMhomocysteine (A+H). The cells were then stimulated with 10 ng/ml TNF for the indicated times, and cytosolic and nuclear fractions were prepared as described in Materials and Methods. (A) Representative western blot showing degradation of IκB-α in cytosolic fraction and translocation of the NFκB subunit p65 to the nucleus in response to TNF. (B) Densitometric analyses of western blots from 3 independent experiments performed as in (A).