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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Alcohol Clin Exp Res. 2013 Nov 13;38(4):889–896. doi: 10.1111/acer.12315

Fig. 5.

Fig. 5

The phosphorylated form of p65 does not accumulate in the nucleus of Ado+Hcy-treated cells. Cells were pre-treated for 2 hours with medium alone (DMEM) or 500 µM adenosine and homocysteine (A+H), then stimulated with 10 ng/ml TNF for the indicated times. Cytosolic and nuclear fractions were prepared, and equal amounts of total protein (15 µg) were analyzed by western blotting.