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. 2014 May 1;15(1):328. doi: 10.1186/1471-2164-15-328

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© Pottorff et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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A. Hbs-1 in the IT84S-2246 x TVu14676 population. The major locus for the heat-induced browning of seed coats phenotype was mapped using datasets from two experiments. Hbs-1 spanned 49.51 cM to 50.69 cM on linkage group 9. SNP marker 1_0032 was the most significant marker for both experiments accounting for 28.3% and 34.1% of the phenotypic variance and is highlighted in red on the linkage group. The significance threshold is indicated by the horizontal broken line. B. Hbs-3 in the IT84S-2246 x TVu14676 population. The minor locus for the heat-induced browning phenotype was mapped using two experimental datasets (Interval Mapping analysis shown). Hbs-2 spanned 8.67 cM to 20.97 cM on linkage group 3. SNP markers 1_0280, 1_1534 and 1_1404 shared the same marker bin, accounting for 6.2% and 6.8% of the phenotypic variance and are highlighted in red on the linkage group. The significance threshold is indicated by the horizontal broken line on the graph.