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. 2014 May 1;15(1):329. doi: 10.1186/1471-2164-15-329

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© Cantsilieris et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

The distributions of unrounded CCL3L1 copy number measurements plotted on a histogram from real-time QPCR and MLPA exon 3 assays. A. The real-time QPCR assay demonstrates substantial variation around integer values. Without defined gaps between each of the clusters, assignment of whole copy number is performed by rounding the data to the nearest integer. B. The MLPA assay demonstrates a distribution of 0–6 copies with peaks centered on integer values. Copy number assignment can be performed by the groups of samples to a particular cluster.