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. 2014 May 8;15(1):348. doi: 10.1186/1471-2164-15-348

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© Srivastava et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Evaluation of plant miRNA target prediction tools for identifying true miRNA-mRNA interaction in non-Arabidopsis species. (A) Comparison of ‘precision’ and ‘recall’ of the six plant specific tools to optimize scores for predicting targets in non-Arabidopsis dataset. (B) Comparison of true positive and false positive predictions by 6 tools independently and in-combination. The intersection of psRNATarget and Tapirhybrid (PH, marked with an arrow) delivers the best trade-off between true and false positive rates. Overlap of TP and FN is represented in (C) when the union of Targetfinder and psRNATarget (upper Venn diagram) or the intersection of psRNATarget, Tapirhybrid, and Targetfinder (PHI; lower Venn diagram) of predictions are made. Again, bold and regular numbers represent false positives and true positives respectively.