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. 2014 May 10;15(1):354. doi: 10.1186/1471-2164-15-354

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© Farrell et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of non-mutagenized parent strains: 2F-1-YFP2, Blader RH-ΔHGXPRT (B-RH) and Gubbels RH-ΔHGXPRT (G-RH). (A) Venn diagram of shared and unique SNVs between the three strains and the GT1 reference genome. (B) The incidence of various mutations causing changes in amino acid coding. For 2F-1-YFP2, B-RH, and G-RH the unique SNVs vs GT1 are shown (these correspond to the 54, 66, and 19 SNVs in panel A, respectively). “RH-srd.” refers to all SNVs shared between B-RH and G-RH and “all-srd.” refers to the SNVs shared between all three lines (these correspond to the and 85 and 984 SNVs in panel A, respectively) Syn.: synonymous; non-syn: non-synonymous. * 50 non-coding SNVs map to the apicoplast genome. (C-E) The incidence of the various base pair changes (SNVs) in the mutants and groups as indicated. Note that we used the n-F-P2 reads instead of 2F-1-YFP2 reads in this analysis since the quality is much higher (Figure 2); ENU specific n-F-P2 mutations were removed from the comparative analysis.