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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Neurobiol Aging. 2014 Feb 15;35(8):1792–1800. doi: 10.1016/j.neurobiolaging.2014.02.012

Fig. 1.

Fig. 1

Astrocyte-derived exosomes promote aggregation and decreased uptake by mixed glial cultures of Aβ1-42 in vitro. (A) HFIP-treated human Aβ1-42 (20 µM) was incubated in 100 µL TBS (pH 7.5) alone (column A) or in the presence of exosomes (12–15 µg protein) with normal rabbit IgG (4 µg/mL; column A+E) or anti-ceramide (4 µg/mL; A+E+antiCer). Samples were centrifuged at 20,000×g after 18 h incubation at 37°C, and resulting pellets were washed and processed for Aβ1-42-specific ELISA. Data reported as mean ± SEM, n=3, *p < 0.01, ANOVA with Bonferroni post hoc test. (B) HFIP-treated human Aβ1-42 was incubated in serum-free DMEM alone (A) or with exosomes, 5 µg (A+1E), 10 µg (A+2E), or 15 µg (A+3E) protein, for 1 h prior to addition to mixed glial cultures for 18 h (final Aβ, 0.5 µM). Cell lysates were subjected to Aβ1-42-specific ELISA. Data are reported as fold-change based on mean ± SEM intracellular pg Aβ1-42/mg protein, n=3, *p < 0.01, ANOVA with Bonferroni post hoc test.