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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Neurobiol Aging. 2014 Feb 15;35(8):1792–1800. doi: 10.1016/j.neurobiolaging.2014.02.012

Fig. 2.

Fig. 2

Injection of exosomes into 5xFAD mouse brains stimulates aggregation of Aβ1-42. (A-D) Confocal micrographs of 5xFAD mouse brain sections following sham injection (A, control) or injection of astrocyte-derived exosomes (B,D) performed on 10-day old 5xFAD pups that were sacrificed 48 h later. Exosomes were harvested from astrocytes pre-labeled with Vybrant CM-DiI (B,D, red) and resuspended in PBS. Control injections (A) contained 0.01% Vybrant CM-DiI in PBS. All sections were labeled with rabbit anti-Aβ1-42 and AlexaFluor488- anti rabbit IgG (green). Nuclei were labeled with DAPI (blue). Note that sham-injected (A) and the area contralateral (C) to the exosome injection site (D) do not show an accumulation of Aβ1-42. (E-F) Positive and negative controls, respectively, are represented by an adult 5xFAD brain (E) and an uninjected 10-day old 5xFAD pup brain (F). Scale bars = 50 µm.