Fig. 4.
PKD1 mediates S1P induced cell survival and SSH1L knockdown mimics S1P protection in response to H2O2 treatment in cardiomyocytes. (A through C) Cardiomyocytes were transfected with Ctr siRNA or PKD1 siRNA. (A) Representative images of cardiomyocytes stained with Calcein (green) and propidium iodide (red) following indicated treatments. Scale bar, 40 μm. Quantification of cardiomyocyte viability (B) and LDH release (C) following the indicated treatments. (D and E) Cardiomyocytes were transfected with Ctr siRNA or SSH1L siRNA. Quantification of cardiomyocyte viability (D) and LDH release (E) following the indicated treatments. n = 6 experiments per treatment for (B) to (E).