Skip to main content
. Author manuscript; available in PMC: 2014 Dec 17.
Published in final edited form as: Sci Signal. 2013 Dec 17;6(306):ra108. doi: 10.1126/scisignal.2004405

Fig. 5.

Fig. 5

S1P or SSH1L knockdown decreases cytochrome c release and preserves mitochondrial membrane potential in response to H2O2 treatment in cardiomyocytes. (A and B) Cytochrome c (Cyto c) release in the cytosolic fraction with H2O2. (A) Representative blots (top) and quantification (bottom) of Cyto c release with or without S1P pretreatment. (B) Representative blots (top) and quantification (bottom) of Cyto c release in Ctr siRNA or SSH1L siRNA transfected cells. (C and D) TMRE fluorescence intensity in response to H2O2 treatment. (C) Representative images of TMRE staining (top) and quantification (bottom) of TMRE fluorescence intensity with or without S1P pretreatment. Scale bar, 20 μm. (D) Representative images of TMRE staining (top) and quantification (bottom) of TMRE fluorescence intensity in Ctr siRNA or SSH1L siRNA transfected cells. Scale bar, 20 μm. n = 6 experiments per treatment for (A) to (D).