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The graph shows averages of values ± s.e.m. from two to four independent transient transfection experiments for each XMRF4a construct, except for 4.3-kb which is a single datum. Each experimental value is the average of two separate C2C12 cell transfections, expressed as the ratio of test construct to RL-SV40 control, normalized to the value obtained for a positive control construct.
