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. 2014 May 27;9(5):e97094. doi: 10.1371/journal.pone.0097094

Figure 2. Effect of JNK-1 inhibition in miR-21 and PDCD4 expression.

Figure 2

A2780CP20 cells were treated with 10 µM SP600125. (A) Western blot shown the inhibition of p-c-Jun after treatment with SP600125 in A2780CP20 cells compared to control (DMSO). (B) SYBR-I-based real-time PCR was performed to calculate the relative pre-mir-21 expression in A2780CP20 cells after treatment with SP600125 inhibitor. (C) Western blot and densitometric analysis of PDCD4 protein expression levels after treatment of A2780CP20 cells with SP600125. (D) PDCD4 protein expression levels after transfection of A2780CP20 with miR-21 oligonucleotide inhibitor. (E) A2780 CP20 cells were transiently transfected with two c-Jun-targeted siRNAs as described in the “Materials and Methods” section. Western blot analysis shows that both c-Jun-siRNAs decreased the c-Jun levels. SYBR-I-based real-time PCR was performed (see “Materials and Methods” section) to calculate the relative pre-mir-21 expression levels in A2780CP20 cells after siRNA-mediated c-Jun silencing. *p<0.05, **p<0.01, ***p<0.001 compared to control. Columns represent the means of triplicates ± S.E.M.