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. 2014 May 27;9(5):e97120. doi: 10.1371/journal.pone.0097120

Figure 1. Expressional profile of OsRACK1A genes (A) and proteins (B) in selected transgenic rice lines.

Figure 1

A, OsRACK1A expression was monitored in seedlings of wildtype (non-transgenic lines, NTL), OsRACK1A over-expressed transgenic lines (OeTL), anti-sense transgenic lines (AsTL) and RNA-interfered transgenic lines (RiTLs), respectively. Relative expression levels were calculated and normalized with respect to OsActin7 (LOC_Os11g06390). Results shown are means of three biological replications±SE. Asterisks (*) indicate significant difference (P<0.05) between the levels of expression of transgenic lines compared with the wildtype (NTL). B, OsRACK1A expression was analyzed by incubating isolated proteins with polyclonal antibodies against OsRACK1A or OseEF1-α (as reference).