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. 2014 May 27;9(5):e97120. doi: 10.1371/journal.pone.0097120

Figure 8. Comparisons of total amylase activity and of expression of two key amylase genes, RAmy1A and RAmy3D, in imbibed seeds of different transgenic lines and the effects of ABA.

Figure 8

A, For amylase activity assay, frozen seeds (20 grains) were homogenized on ice and 100 µl aliquot of supernatant was taken into the assay after centrifugation. The reaction product was monitored using 3,5-dinitrosalicylic acid (DNS) method. B, C and D, Expressions of RAmy1A and RAmy3 were monitored in seeds of NTL, OeTL, AsTL and RiTL, respectively after 48h of imbibition in water or in ABA (20 µM). Relative expression levels were measured by qRT-PCR and normalized with respect to OsActin7 (LOC_Os11g06390). Results shown are means of three biological replications±SE. Asterisks (*) indicate significant difference (P<0.05) between the levels of expression of transgenic lines compared with the NTL.