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. 2014 May 27;9(5):e96470. doi: 10.1371/journal.pone.0096470

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© 2014 Greco et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Genomic DNA (∼0.5 µg), after RNase treatment, isolated from strains of E. siliculosus (RHO12, LIA4A; REP10–11; EC524) using initial biomass of 25, 50 and 100 mg. (B) The quality of isolated DNA was confirmed by electrophoresis analysis of a DNA PCR product using an alpha tubuline (TUA) housekeeping gene. (C) Electrophoretic analysis of EcoRV enzyme digestion product of genomic DNA confirms that the extracted DNA is suitable for downstream application (gels stained with ethidium bromide). M: 100-bp, 1-Kb DNA and High Range RNA Ladder (Fermentas, Italy).