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. 2014 May 27;9(5):e98295. doi: 10.1371/journal.pone.0098295

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© 2014 Pallante et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Quantitative RT-PCR analysis was performed to analyze the expression levels of CBX7-regulated genes in cbx7^+/+ and cbx7^-/- MEFs. Values are expressed as Relative expression with respect to the cbx7^+/+, that was set equal to 1. A) Fos, fosb and egr1 were less expressed in cbx7^-/- MEFs compared to the cbx7^+/+ MEFs. B) On the contrary, spp1, spink1 and steap1 genes were more expressed in MEFs cbx7^-/- compared to the MEFs cbx7^+/+. C) Cbx7^-/- MEFs were transiently transfected with a mammalian vector expressing mouse cbx7 (myc-His-tagged) mRNA (cbx7^-/—R). Restoration of cbx7 expression is able to revert the phenotype of the cbx7^-/- MEFs (A, B). Values are expressed as Relative expression with respect to the cbx7^+/+, that was set equal to 1. D) The expression of fos and egr1 proteins was evaluated by western blot in MEFs obtained from cbx7^-/- mice in comparison to cbx7^+/+ MEFs. β-Actin expression was evaluated to normalize protein loading.