Figure 2. Let-7a, miR-9 and miR-302a primary transcripts are differentially processed in undifferentiated and differentiated extracts from P19 cells.
Internally radiolabeled primary transcripts (50 × 103 c.p.m. (counts per minute), approximately 20 pmol) were incubated in the presence of either d0 (Lanes 1) or d9 (Lanes 2) P19 cell extracts. The products were analyzed on an 8% denaturing polyacrylamide gel. [M] – RNA size marker. (*) – Nonspecific in vitro processing products. Pri-miR-101 processing served as a control. The results are representative of at least three independent experiments.