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. 2014 May 27;9(5):e98277. doi: 10.1371/journal.pone.0098277

Figure 5. Voltage-dependent currents in the depolarizing range.

Figure 5

Sodium currents, triggered at −30 mV in voltage clamped VE neurons were completely blocked by 0.5 µM TTX (A) and displayed systematic voltage dependency (B). Sustained outward currents, evoked by 10 mV, 1500 ms depolarizing steps from a holding potential of −60 mV (A), were partially blocked by 0.5 mM TEA in representative VE (C) and LOC (E) neurons. The sustained current was quantified at the last 100 ms of the depolarization before and after TEA application and plotted against the voltage in the VE (D) and in the LOC (F) neuron. The TEA-sensitive component was calculated by subtracting the TEA current from the control current in the respective example neuron (D and F). Transient and sustained outward currents, evoked under influence of TTX and TEA in a VE neuron by depolarizing 10 mV, 500 ms steps, preceded by a −100 mV, 50 ms hyperpolarization step to de-inactivate potential low-voltage activated currents, were partially blocked by 0.4 mM 4-AP (G). The 4-AP-sensitive component was calculated and plotted as above (H).