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. 2014 May 27;9(5):e97864. doi: 10.1371/journal.pone.0097864

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© 2014 Nieminen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Metabolic activities measured by XTT-assay (panel A, B) and dsDNA levels (panel C, D) reflecting the biomass of C. albicans biofilms at pH 5.2 and 7.4. Biofilms were grown for 4, 24 or 48 h in RPMI at pH 7.4 and then exposed to PBS, caspofungin, HICA, leucine, cysteine or ethanol for another 24 h. Values were measured twice in triplicate and expressed as mean (±SEM). Means were compared to the control treatment (RPMI). Statistically significant differences were calculated using a GEE-model and were marked (**p<0.001,* 0.001<p<0.05).