Skip to main content
. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: Gastroenterology. 2014 Feb 13;146(7):1739–1751.e14. doi: 10.1053/j.gastro.2014.02.005

Figure 3.

Figure 3

Effect of inhibition of EGFR on levels of SMOX and DNA damage in gastric epithelial cells. Imst cells were pretreated with the EGFR inhibitor AG1478 (150 nM) for 45 min and co-cultured with H pylori 7.13. (A) After 6 h, levels of SMOX mRNA were measured by real-time PCR. (B) After 24 h, levels of SMOX protein were measured by Western blotting and quantified by densitometry. (C) Levels of SMOX protein after 24 h by flow cytometry. (D) Representative histogram for SMOX. (E) DNA damage measured after 24 h by flow cytometry for 8-oxoguanosine. (F) Representative histogram for 8-oxoguanosine. For A, ***P < .001 vs DMSO control, §§§P <.001 vs DMSO + 7.13; for B, C, and E, ***P < .001 vs DMSO, §§§P <.001 vs DMSO + 7.13. Data are from 3 experiments in duplicate.