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. Author manuscript; available in PMC: 2015 May 22.
Published in final edited form as: Chem Biol. 2014 Apr 10;21(5):636–646. doi: 10.1016/j.chembiol.2014.02.019

Figure 3.

Figure 3

Cycloaddition assay for KirCII:ACP interactions. (A) KirCII-catalyzed trans-acylation and subsequent cycloaddition. (B) In-gel fluorescence detection of a series of KirCII control assays. Coomassie Blue image shows ACP loading in each reaction. (C) Fluorescence intensity of labeled ACP5Kir in a series of control reactions using various trans-ATs or no enzyme. (D) Fluorescence intensity of KirCII-labeled ACP5Kir as a function of time. (E) Fluorescence intensity of labeled ACP5Kir as a function of KirCII concentration. Error bars represent the standard deviation of the mean (n=3)