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. Author manuscript; available in PMC: 2015 May 22.
Published in final edited form as: Chem Biol. 2014 Apr 10;21(5):636–646. doi: 10.1016/j.chembiol.2014.02.019

Figure 5.

Figure 5

Mapping the KirCII:ACP interaction epitope by alanine scanning mutagenesis. (A) Trans-acylation rates of holo-ACP5Kir alanine mutants with KirCII. Rates are expressed as a percentage of the activity with wild-type ACP5Kir. Wild-type positions that were Ala/Gly were not mutated. Mutants that displayed <20% the activity of the wild-type holo-ACP5Kir are highlighted red. (B) Trans-acylation activities of ACP5Kir alanine mutants mapped onto an ACP5Kir homology model ribbon diagram (left) and its computed surface (right). Red, <20% activity; green, 20–80% activity; yellow, >80% activity; grey, not determined. Error bars represent the standard deviation of the mean (n=3).