Peripheral blood mononuclear cells from healthy donor volunteers were treated with LPS and G6-NH2 alone or in combination for 24 h. (A) PS presentation on the surface of cells incubated with increasing concentrations of G6-NH2 dendrimers; (i) 4, (ii) 8, (iii) 10 and (iv) 25 µg/ml. (B) TF expression on the surface of cells (i) untreated or incubated with (ii) 1 ng/ml LPS or (iii) 4 µg/ml of G6-NH2 dendrimers. (C) TF expression on the surface of cells treated with 1 ng/ml LPS or 4 µg/ml G6-NH2 dendrimers alone or in combination (LPS + G6-NH2). Shown is the mean value ± standard deviation of percentage-positive cells derived from analysis of ten donor specimens. PC was assigned a 100% value. (D) PS presentation on the surface of cells treated with 1 ng/ml LPS or 10 µg/ml G6-NH2 dendrimers alone or in combination (LPS + G6-NH2). Shown is the mean value ± standard deviation of percentage-positive cells derived from analysis of six donor specimens. *p < 0.05.
FITC: Fluorescein isothiocyanate; G6-NH2: Generation 6 amine-terminated polyamidoamine dendrimer; LPS: Lipopolysaccharide; NC: Negative control (cell culture media); PC: Positive control (10 µg/ml LPS); PE: Phycoerythrin; PS: Phosphatidyl serine; SSC: Side scatter; TF: Tissue factor.