Platelet-containing and platelet-free PBMCs from healthy donor volunteers were treated with 1 ng/ml of LPS or G6-NH2 alone (LPS and G6-NH2, respectively) or in combination (LPS + G6-NH2) for 24 h. (A) TF expression and (B) phosphatidyl serine presentation were analyzed by flow cytometry. TF was assessed using 4 µg/ml G6-NH2. Phosphatidyl serine was assessed using 4, 8 and 10 µg/ml G6-NH2. Shown is the mean percentage of positive cells. Shown are data from a single donor. Data from additional donors are provided in Supplementary Figure S5.
LPS: Lipopolysaccharide; G6-NH2: Generation 6 amine-terminated polyamidoamine dendrimer; NC: Negative control; PBMC: Peripheral blood mononuclear cell; PC: Positive control; TF: Tissue factor.