Skip to main content
. Author manuscript; available in PMC: 2014 Sep 11.
Published in final edited form as: Nanomedicine (Lond). 2013 Nov 27;9(9):1311–1326. doi: 10.2217/nnm.13.137

Figure 6. Role of platelets in enhancement of endotoxin-induced procoagulant activity by amine-terminated dendrimers.

Figure 6

Platelet-containing and platelet-free PBMCs from healthy donor volunteers were treated with 1 ng/ml of LPS or G6-NH2 alone (LPS and G6-NH2, respectively) or in combination (LPS + G6-NH2) for 24 h. (A) TF expression and (B) phosphatidyl serine presentation were analyzed by flow cytometry. TF was assessed using 4 µg/ml G6-NH2. Phosphatidyl serine was assessed using 4, 8 and 10 µg/ml G6-NH2. Shown is the mean percentage of positive cells. Shown are data from a single donor. Data from additional donors are provided in Supplementary Figure S5.

LPS: Lipopolysaccharide; G6-NH2: Generation 6 amine-terminated polyamidoamine dendrimer; NC: Negative control; PBMC: Peripheral blood mononuclear cell; PC: Positive control; TF: Tissue factor.