. Author manuscript; available in PMC: 2014 Sep 11.

Published in final edited form as: Nanomedicine (Lond). 2013 Nov 27;9(9):1311–1326. doi: 10.2217/nnm.13.137

Table 1.

Summary of dendrimer characterization.

Surface	Generation	Hydrodynamic diameter (nm)	Zeta- potential (mV)	Endotoxin (EU/ml/100 µg)
Amine (−NH₂)	3	4.1 ± 0.1	55.7 ± 0.7	<0.05
	4	5.1 ± 0.1	61.9 ± 1.5	<0.05
	5	6.5 ± 0.2	56.5 ± 0.7	<0.05
	6	8.3 ± 0.1	67.1 ± 2.4	<0.05

Carboxy (−COOH)	5	7.7 ± 0.3	−13.3 ± 1.9	7.20

Hydroxy (−OH)	5	6.6 ± 0.1	17.9 ± 0.5	<0.05

Guanidine	5	8.1 ± 2.5	45.0 ± 3.2	<0.50

Dendrimer hydrodynamic diameter (dynamic light scattering; intensity peak), zeta-potential and endotoxin contamination were analyzed as described in the ‘Materials & methods’ section. The hydrodynamic diameter and zeta-potential data are ± standard deviation. In the limulus amebocyte lysate assay, dendrimers were tested at 1:5, 1:50 and 1:500 dilutions; shown are the data adjusted for the dilution factor.