Table 1.
Surface | Generation | Hydrodynamic diameter (nm) |
Zeta- potential (mV) |
Endotoxin (EU/ml/100 µg) |
---|---|---|---|---|
Amine (−NH2) | 3 | 4.1 ± 0.1 | 55.7 ± 0.7 | <0.05 |
4 | 5.1 ± 0.1 | 61.9 ± 1.5 | <0.05 | |
5 | 6.5 ± 0.2 | 56.5 ± 0.7 | <0.05 | |
6 | 8.3 ± 0.1 | 67.1 ± 2.4 | <0.05 | |
Carboxy (−COOH) | 5 | 7.7 ± 0.3 | −13.3 ± 1.9 | 7.20 |
Hydroxy (−OH) | 5 | 6.6 ± 0.1 | 17.9 ± 0.5 | <0.05 |
Guanidine | 5 | 8.1 ± 2.5 | 45.0 ± 3.2 | <0.50 |
Dendrimer hydrodynamic diameter (dynamic light scattering; intensity peak), zeta-potential and endotoxin contamination were analyzed as described in the ‘Materials & methods’ section. The hydrodynamic diameter and zeta-potential data are ± standard deviation. In the limulus amebocyte lysate assay, dendrimers were tested at 1:5, 1:50 and 1:500 dilutions; shown are the data adjusted for the dilution factor.