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. 2014 May 28;34(22):7447–7457. doi: 10.1523/JNEUROSCI.0256-14.2014

Figure 2.

Figure 2.

Effect of context-induced renewal of alcohol seeking after punishment-imposed abstinence on Fos expression in LH. A, Outline of the experimental procedure for this experiment. B, Renewal test: Mean ± SEM active and inactive lever presses during testing. *p < 0.05 different from the punishment context group. C, Hypocretin-IR, Fos-IR, and Fos + hypocretin-IR neurons: Number of IR neurons per square millimeter in the LH of rats tested in punishment context B (n = 8), alcohol training context A (n = 8), or taken from the home cage (no-test, n = 5). *p < 0.05, different from the punishment context and no-test groups; #p < 0.05, different from the no-test group. D, Representative photomicrograph of hypocretin and Fos immunohistochemistry. Scale bar, 50 μm. Inset shows high-magnification example of IR neurons. Scale bar, 20 μm.