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. 2014 May 28;34(22):7600–7610. doi: 10.1523/JNEUROSCI.4364-13.2014

Figure 2.

Figure 2.

Actin cytoskeleton regulates CaMKII mobility. A, Histogram of Deff of cells transfected with mEos2-CaMKIIα or mEos2-CaMKIIα/Cer3-CaMKIIβ (1:1) (n = 13 neurons for mEos2-CaMKIIα and n = 17 neurons for mEos2-CaMKIIα/Cer3-CaMKIIβ). B, Histograms of Deff were fit as in Figure 1K, and the proportion of each kinetic subpopulation was quantified. Deff1: 0.0025 μm2/s (9.3 ± 3.3%); Deff2: 0.0173 μm2/s (35.9 ± 5.4%); Deff3: 0.1903 ± 0.045 μm2/s (54.8 ± 4.9%) in the mEos2-CaMKIIα expressing cells (n = 11 neurons). Deff1: 0.0025 μm2/s (16.3 ± 3.6%); Deff2: 0.011 μm2/s (46.3 ± 5.7%); Deff3: 0.161 μm2/s (37.4 ± 3.8%) in the cells transfected with mEos2-CaMKIIα/Cer3-CaMKIIβ (n = 15 neurons). *p < 0.05 (two-way ANOVA repeated measures with one factor repeated). C, Histogram of Deff of cells transfected with mEos2-CaMKIIα/Cer3-CaMKIIβ before and after Lat A (n = 3 neurons). D, Deff distributions before or after Lat A treatment (n = 3 neurons). *p < 0.01 before versus after lat A (two-way ANOVA repeated measures with two factors repeated).