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The spatial distribution of CaMKII mobility is heterogeneous within cells. A, Deff was calculated and color-coded to construct a spatial map of molecular mobility. Scale bar: left top, 1.5 μm. Enlarged view of the spine marked from the left. Scale bar: right top, 1 μm. B, Histogram of Deff from spines or dendrites (K-S test, p < 0.001, n = 5). C, Deff in spines or dendrites was fit as in Figure 1K, and the proportion of each kinetic subpopulation was quantified (n = 4).
