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. 2014 May 28;34(22):7704–7714. doi: 10.1523/JNEUROSCI.4694-13.2014

Figure 7.

Figure 7.

Optically evoked synaptic plasticity is normal for Cre-dependent ChR2 expression in the absence of AAV. A, To express ChR2 in CA3 neurons, Cre-dependent flox-hChR2 mice were either crossed with CaMKII-Cre mice or injected with AAV1-Cre. To control for the mutated variant hChR2 expressed by flox-hChR2 mice, WT mice were also injected with AAV1-hChR2. B, Top, Representative EPSCs evoked by optical trains delivered over axons at 10 and 50 Hz. Bottom, Averaged normalized EPSC amplitudes during trains evoked optically (flox-hChR2 × CaMKII-Cre, n = 7; flox-hChR2 + AAV1-Cre, n = 10; WT+AAV1-hChR2, n = 17) and electrically (WT uninjected mice, n = 18). C, Left, Representative EPSCs from pairs of stimuli delivered at 10–200 ms ISIs. Right, Average paired-pulse ratios for optical (flox-hChR2 × CaMKII-Cre, n = 7; flox-hChR2 + AAV-Cre, n = 18; WT+AAV-hChR2, n = 48) and electrical stimulation (WT uninjected mice, n = 25). The 20 ms data point for WT+AAV-hChR2 was offset laterally to be visible. Data are expressed as average ± SEM.