Table 1.
Design of Kasalath and CG14 allele-specific markers for genotyping diverse genotypes
| Marker name | Allele | Sequence (5′–3′) | Size (bp) | |
|---|---|---|---|---|
| K46-1a | Kas | K46-1 | K46-1 | 523 |
| Fw: TGAGATAGCCGTCAAGATGCT | Rv: AAGGACCACCATTCCATAGC | |||
| K46-K1 | Kas | K46-1 | K46-Ksp3rv | 342 |
| Fw: TGAGATAGCCGTCAAGATGCT | Rv: TGAGCCAGTAGAATGTTTTGAGG | |||
| K46-CG1 | CG | K46-CGsp2fw | K46-1 | 258 |
| Fw: CTAGAGTATCTCCACAGTCGTT | Rv: AAGGACCACCATTCCATAGC | |||
| K46-K2 | Kas | K46-Ksp4fw | K46-Ksp3rv | 433 |
| Fw: CTGAAGTGAAAAGAATGACTAA | Rv: TGAGCCAGTAGAATGTTTTGAGG | |||
| K46-CG2 | CG | K46-CGsp4fw | K46-CGsp3rv | 433 |
| Fw: CCGAAGTAAGAAGAATGACGGA | Rv: TGATCCAGGAGAATGTTTTGTGG | |||
| K46-3 | Kas/CG | K46-3 | K46-3 | 400 |
| Fw: TCCAAAGATCTCTGATTTTGGC | Rv: GCTTTCCAACATCTCAAGGACT | |||
aChin et al. 2011