Fig. 5.

Expression of the resistant allele of Ph-3 in susceptible Moneymaker resulted in resistance to P. infestans. Transgenic lines were tested in two independent experiments. a Non-transformed Moneymaker showed mycelium growing on the infected leaf areas; b transformed Moneymaker expressing Ph-3 showed no symptom and c transformed Moneymaker not expressing Ph-3 showed mycelium growing on the infected leaf areas. It is worthwhile to note that non-transformed Moneymaker plants were grown from seeds and that transformed Moneymaker were from cuttings. Three leaflets in a, b or c were taken from one inoculated leaf and three leaves per plant were tested. Photographs were taken 7 days post-inoculation