Fig. 8.

The level of cytochrome c (a cytoplasmic, b mitochondrial fraction), Ras (c), Raf (d), LC3 (e) and beclin 1 (f) expression with representative blots and the activity of caspase 3, 8, 9 (g) after sorafenib (S) and quercetin (Q) treatment for 48 h in T98G cells transfected with specific siRNA anti-Hsp27 (si27) and anti-Hsp72 (si72). The data were normalised relative to β-actin (not shown). C control cells, SQ simultaneous drug treatment, si27 or si72 specific siRNA blocking Hsp27 or Hsp72 expression, TR transfection reagent, TRsi transfection reagent with specific siRNA, *P < 0.05 compared to control