Table 2.
Results of the qPCR analysis for the insulation materials: calculated copy numbers per ng extracted DNA for the test items and the samples taken from the historical building after 18 and 32 months of installation.
| Material | Climate chamber | Historical building | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Inoculated strains—6 months | 18 months | 32 months | |||||||
| a | b | c | d | Control | 1st floor | 2nd floor | 1st floor | 2nd floor | |
| Sprayed cellulose | 55.47 ± 20.62 | 216.93 ± 25.83 | 87.32 ± 32.33 | 21.68 ± 14.10 | 8.88 ± 5.39 | 1.88 ± 0.95 | 1.84 ± 1.27 | No sample | 3.39 ± 1.06 |
| Bloated Perlite board | 95.26 ± 14.28 | 167.19 ± 17.04 | 156.22 ± 109.96 | 82.83 ± 12.53 | 7.98 ± 0.95 | 0.88 ± 0.33 | 2.85 ± 1.99 | 8.75 ± 2.10 | 11.82 ± 10.51 |
| Bloated Perlite plaster | 5.76 ± 0.14 | 20.38 ± 6.61 | 6.04 ± 5.64 | 27.47 ± 13.18 | 15.93 ± 4.17 | 5.75 ± 5.35 | 4.41 ± 1.21 | 3.85 ± 0.92 | 15.70 ± 1.05 |
| Wooden soft-board | 4174.16 ± 170.36 | 5480.56 ± 569.09 | 1092.86 ± 118.93 | 906.79 ± 139.36 | 25.13 ± 11.28 | 36.95 ± 12.89 | 73.10 ± 23.79 | 19.94 ± 1.40 | 317.33 ± 39.12 |
| Reed board with loam | 29.19 ± 3.78 | 35.48 ± 19.46 | 25.80 ± 11.55 | 21.59 ± 3.01 | 3.60 ± 1.91 | 19.59 ± 3.04 | 91.90 ± 11.65 | 92.51 ± 22.90 | 112.34 ± 30.54 |
Copy number values written in italics are below the detection limit (30.57 copies/ng extracted DNA) of the qPCR assay. (a) MA 1610 Cladosporium cladosporioides, (b) MA 1615 Aspergillus niger, (c) MA 1701 Penicillium chrysogenum, (d) mixture of all three fungal spore suspensions.