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. 2014 May 23;7:77. doi: 10.1186/1754-6834-7-77

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Copyright © 2014 Kadić et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Volume-based particle-size distribution during agitation and subsequent enzymatic hydrolysis. Material used: spruce at 13% WIS. (A) Agitation for 48 hours at 600 rpm followed by 48 hours of agitation at 100 rpm. (B) Agitation for 96 hours at 100 rpm. (C) Agitation for 48 hours at 600 rpm followed by hydrolysis for 48 hours at 100 rpm (only hydrolysis shown). (D) Agitation for 48 hours at 600 rpm followed by hydrolysis for 48 hours at 600 rpm (only hydrolysis shown). Markers: dash line represents 0 hours, two dash line represents 24 hours, filled circles represents 48 hours, x represents 50 hours, and empty circles represents 96 hours.