Table 1.
Enzymatic activities identified by screening the ikaite strain collection
| Activity | Isolates | No. of 16S sequences | Closest relative (16S rDNA % identity) | Class |
|---|---|---|---|---|
| Phosphatase |
102 |
|
|
|
| α-Galactosidase |
75 |
|
|
|
| Protease |
73 |
|
|
|
| α-Amylase |
57 |
|
|
|
| β-Galactosidase |
25 |
|
|
|
| β-Glucanase |
24 |
|
|
|
| Cellulase |
1 |
|
|
|
| β-Xylanase |
1 |
|
|
|
| β-Mannanase |
0 |
|
|
|
|
Total |
358 |
|
|
|
|
Groups of activity |
Total no. |
|
|
|
| α-Galactosidase |
45 |
3 |
Loktanella vestfoldensis (98-100%) |
α-Proteobacteria |
| Phosphatase + protease |
32 |
5 |
Arsukibacterium ikkense (98-100%) |
γ-Proteobacteria |
| Phosphatase |
29 |
4 |
Pseudomonas sabulinigri (99%) |
γ-Proteobacteria |
| |
|
1 |
Loktanella vestfoldensis (100%) |
α-Proteobacteria |
| |
|
1 |
Stenotrophomonas rhizophila (100%) |
γ-Proteobacteria |
| |
|
1 |
Arsukibacterium ikkense (100%) |
γ-Proteobacteria |
| |
|
1 |
Idiomarina fontislapidosi (95%) |
γ-Proteobacteria |
| α-Galactosidase + β-galactosidase |
20 |
2 |
Natronobacillus azotifigens (98%) |
Bacilli |
| |
1 |
Rhodonellum psychrophilum (100%) |
Bacteroidetes |
|
| |
1 |
Klebsiella pneumoniae (95%) |
γ-Proteobacteria |
|
| α-Amylase |
17 |
1 |
Pseudomonas poae (100%) |
γ-Proteobacteria |
| |
|
1 |
Marinovum algicola (100%) |
α-Proteobacteria |
| |
|
1 |
Thermoleophilum minutum (100%) |
Actinobacteria |
| |
|
1 |
Rhodobacter veldkampii (96%) |
α-Proteobacteria |
| Phosphatase + α-amylase + protease + β-glucanase |
17 |
9 |
Arsukibacterium ikkense (98-100%) |
γ-Proteobacteria |
| Phosphatase + α-amylase + protease |
10 |
7 |
Arsukibacterium ikkense (100%) |
γ-Proteobacteria |
| |
|
1 |
Pseudomonas sabulinigri (99%) |
γ-Proteobacteria |
| Protease |
8 |
2 |
Pseudomonas sabulinigri (96-97%) |
γ-Proteobacteria |
| |
|
1 |
Aquimonas voraii (93%) |
γ-Proteobacteria |
| Phosphatase + α-amylase |
7 |
5 |
Arsukibacterium ikkense (98-100%) |
γ-Proteobacteria |
| Phosphatase + protease + β-glucanase |
7 |
4 |
Arsukibacterium ikkense (98-100%) |
γ-Proteobacteria |
| |
|
1 |
Klebsiella variicola (97%) |
γ-Proteobacteria |
| α-Galactosidase + β-galactosidase + protease |
3 |
3 |
Rhodonellum psychrophilum (96-99%) |
Bacteroidetes |
| α-Galactosidase + β-galactosidase + α-amylase |
2 |
2 |
Rhodonellum psychrophilum (95-99%) |
Bacteroidetes |
| α-Galactosidase + α-amylase |
2 |
2 |
Loktanella vestfoldensis (100%) |
α-Proteobacteria |
| α-Galactosidase + α-amylase + protease |
1 |
1 |
Loktanella vestfoldensis (100%) |
α-Proteobacteria |
| α-Galactosidase + phosphatase |
1 |
1 |
Loktanella vestfoldensis (100%) |
α-Proteobacteria |
| α-Galactosidase + cellulase + β-xylanase |
1 |
1 |
Demequina aestuarii (98%) |
Actinobacteria |
| α-Amylase + protease |
1 |
1 |
Arsukibacterium ikkense (100%) |
γ-Proteobacteria |
| Total | 203 | 65 |
A total of 322 cultured isolates were screened for 9 different enzyme activities at pH 10 and 10, 20 and 28°C. The closest relative was determined for selected isolates by partial sequencing of the 16S rRNA gene.