. 2014 May 20;13:72. doi: 10.1186/1475-2859-13-72

Table 6.

Comparison of β-galactosidases

	BGal_I17E2	BGal_I3H5	*A. ikkensis* β-galactosidase	*E. coli* LacZ
Monomeric size	455aa	820aa	1,041aa	1,029aa
Monomeric size	52.75 kDa	92.25 kDa	119.13 kDa	116.97 kDa
GH family	1	2	2	2
Closest relatives	Clostridium hathewayi (53% identity) [GenBank:WP_006771328]	Roseburia hominis (51% identity) [GenBank:YP_004838333]	-	-
Arg	3.3%	4.5%	4.0%	6.4%
Arg/Arg + Lys ratio	0.31	0.44	0.42	0.77
Pro	3.7%	3.3%	4.8%	6.1%
Active site	WFAEYTKVM	LVTEYNGHM	ILCEFSHAM	ILCEYAHAM

Comparison of the β-galactosidases from the two library clones, IKA17E2 (BGal_I17E2) and IKA3H5 (BGal_I3H5), to β-galactosidases from the psychrotrophic A. ikkensis[45] and the mesophilic E. coli (LacZ) [48]. Active site glutamic acid (E) highlighted in bold.